The Biodiverse Food Study: Investigating the Impact of Nutritional Diversity on Human Health, Soil Regeneration, and Ecosystem Resilience

Abstract

Biodiversity in food systems has been largely overlooked in modern agricultural and nutritional science, despite emerging evidence suggesting that dietary diversity is critical for human health, microbiome optimization, and environmental sustainability. This study investigates the impact of biodiverse food consumption on human physiological performance, gut microbiota composition, immune function, and metabolic health, while simultaneously analyzing the effects of high-diversity cropping systems on soil microbial activity, carbon sequestration, and ecosystem resilience. By integrating methodologies from nutritional biochemistry, soil microbiology, regenerative agriculture, and ecological network theory, this research aims to quantify the systemic benefits of nutritional diversity-driven food systems.

Introduction

The prevailing global food system is heavily reliant on monoculture production, a practice that has led to declining soil health, nutrient depletion in crops, loss of biodiversity, and increased susceptibility to climate-related shocks. Concurrently, the human diet has become increasingly homogenized, with over 75% of the world’s food supply derived from only 12 plant and 5 animal species (FAO, 2020). This lack of dietary diversity has been linked to gut dysbiosis, metabolic disorders, and reduced immune resilience. The Biodiverse Food Study seeks to address this knowledge gap by investigating how dietary diversity, when based on regenerative and biodiverse agricultural models, influences human health markers, microbiome stability, and ecosystem services.

Research Objectives

1. To assess the impact of biodiverse dietary intake on human microbiome diversity, metabolic function, and immune modulation.
2. To analyze soil health improvements associated with diverse, polycultural farming systems compared to conventional monoculture-based agriculture.
3. To investigate the ecological benefits of integrating multi-species agroecosystems, including pollinator resurgence, increased soil organic matter, and enhanced climate resilience.
4. To explore the potential of biodiverse diets in mitigating chronic diseases, improving cognitive function, and enhancing physiological endurance.

Methodology

1. Human Nutritional Study Design

A longitudinal, controlled dietary intervention study will be conducted to evaluate the effects of a high-biodiversity diet on gut microbiota composition and systemic health markers. Participants (n=200) will be stratified into:

• Biodiverse Diet Group (BDG): Consuming a diet composed of 150+ plant species, heirloom grains, probiotic-rich foods, and sustainable animal proteins.
• Standard Western Diet Group (SWDG): Following a conventional diet based on common supermarket food items with low phytonutrient and microbial diversity.

Biometric and biochemical markers analyzed:

• Gut microbiome sequencing (16S rRNA analysis)
• Inflammatory cytokine profiling
• Blood glucose, lipid panel, and hormonal fluctuations
• Cognitive function assessment (neurotransmitter analysis, EEG biomarkers)

2. Soil Health & Agricultural Systems Study

Parallel to the human trial, a comparative agroecological study will be conducted across three different land-use systems:

• Monoculture Farming (Control): Standard industrial crop production.
• Organic Polyculture (OP): Mixed-crop regenerative farming without synthetic inputs.
• Nutritional Diversity Agroecosystem (NDA): High-density multi-species agriculture integrating nitrogen-fixing plants, root microbiome inoculation, and biomimetic soil amendment techniques.

Soil health parameters measured:

• Microbial biodiversity index (metagenomic sequencing of soil microbiota)
• Soil organic carbon (SOC) accumulation and sequestration rates
• Fungal-to-bacterial ratio as an indicator of soil health stability
• Soil respiration rates and enzymatic activity markers

3. Ecological Impact Study

Using remote sensing, drone-assisted ecological mapping, and biodiversity indexing, the study will assess:

• Pollinator abundance and diversity (native bees, butterflies, and other beneficial insects).
• Wildlife corridor effectiveness in areas with biodiverse agroforestry integration.
• Water retention capacity of biodiverse vs. monoculture plots.
• Carbon sequestration potential of tree-based intercropping systems.

Expected Outcomes & Hypothesis

This study hypothesizes that:

1. A biodiverse diet will lead to higher gut microbial diversity, improved metabolic resilience, and stronger immune function compared to a conventional diet.
2. Regenerative, biodiverse farming practices will show significantly higher soil microbial activity, enhanced carbon sequestration, and improved nutrient cycling versus conventional monocultures.
3. Ecosystem services (pollinator diversity, soil fertility, climate resilience) will be positively correlated with the degree of biodiversity in agroecosystems.
4. The integration of biodiverse diets into human nutrition models could contribute to a paradigm shift in public health, reducing the incidence of chronic disease while fostering environmental regeneration.

Significance of Study

The Biodiverse Food Study is the first of its kind to holistically evaluate the interconnected relationship between human health, soil microbiology, and agroecological resilience through the lens of nutritional diversity. The findings could provide a new framework for policy recommendations, dietary guidelines, and regenerative food system development aimed at addressing the global challenges of nutrient depletion, food insecurity, and ecological degradation.

Funding & Implementation

To support the research, we seek multi-sector collaboration involving:

• Academic Institutions & Research Centers (for clinical trials and microbiome sequencing).
• Regenerative Agriculture Networks (for field implementation and ecosystem monitoring).
• Technology Partners (for AI-driven soil analysis, blockchain-based biodiversity tracking, and drone-assisted reforestation).
• Government & International Development Agencies (for policy integration and land-use reform strategies).

Conclusion

By bridging the disciplines of human nutrition, soil science, regenerative agriculture, and ecological systems analysis, the Biodiverse Food Study aims to demonstrate that diverse, nature-aligned food systems can simultaneously heal people and the planet. With escalating climate instability, biodiversity loss, and metabolic disease epidemics, the imperative to integrate nutritional diversity into global food systems has never been greater. This study serves as a blueprint for a regenerative future—one where food is not just sustenance, but a tool for biological performance, ecological healing, and human resilience.

Key References

• FAO (2020). The State of Food Security and Nutrition in the World. United Nations.
• Montgomery, D. (2017). Growing a Revolution: Bringing Our Soil Back to Life. Norton.
• Sonnenburg, J. & Sonnenburg, E. (2019). The Good Gut: Taking Control of Your Weight, Your Mood, and Your Long-term Health. Penguin Random House.
• Bender, S.F., Wagg, C., & van der Heijden, M.G. (2016). An Underground Revolution: Biodiversity and Soil Microbial Communities for Agricultural Sustainability. Trends in Ecology & Evolution, 31(6), 440-452.
• Addressing the climate change and poverty nexus – FAO ISBN:9251319561, 9789251319567

FUN FUTURE?

We are still socially and culturally plagued by this idea that we will oversee, and have a uniform plan for the whole world, ripping individuality and mono-cropping everything from the plants to the people.

There is a small business opportunity in small agriculture that can literally be performed anywhere in the world right now. The information that is run across the modern human eyeballs and brain stems is anything but the simple truth right in front of us.

It may sound like crazy talk.

Nature right now offers plenty of keys, we just don’t take them. We don’t take the time to learn to use herbal and primary forest elements that grow all on their own. We want what we want and too many times, what we want is bad for us. Many of those times we can claim we didn’t know. Whatever, in the end, what is most important is when we finally learn to do things the right way, or just not do them. There is a limitless benefit to going with the teaching of nature, whereas doing things wrong carries huge liabilities and risks not necessary for excellent success.

My father mentioned to me once, that it was surprising “how people could not add up that they can make millions legitimately but will risk it all and do something unethical, to make a million point one.” That always sticks out in my head when I see it, and I mostly see it in a ridiculously compounded stack of shady extra profit-making moves that companies have to lie about later and I see it with the epidemic of child kidnapping and brainwashing across the U.S. we have now clinically labeled (lied about), as ‘parental alienation.’

We are all fighting the selfish disease and thinking we own some land, completely dismissing we did not create it, it was gifted to us by something that could create it, and it is rich and beautiful. We war over it, in its name kill, not for food or for any justifiable reason but for the insanity that is completely foreign to this world and nature itself; that we somehow create the environment, the food, and can charge a paper or digit that control us for this creation. A lie within a lie within a lie, and then some liar evaluation at the end of it all – guaranteed only a measurement of how much more lying is needed since there does not seem to be any mention of education before the test?!

Whatever the intention behind the discussion, I would like to offer a picture of how better nutrition can lead us towards, better business and better sustainability, and most importantly better health for us and the ecology.

I would like to offer it in words the private sector can resonate with.

Small Nutritionally Diverse Cafe & Market

Start small, maybe keep it small, the starting place small business model for the Nutritional Diversity Diet.

The Biodiverse or Nutritionally Diverse Cafe & Restaurant concept is great because it is a place where the family can go not just nourish their bodies with better, correct nutrition plans and menu items, but it is also a great way to learn about key principles in biological life, healthy living, basic correct hydration, and nature-based agriculture – things very vital to optimal performance.

A new experience in dining, one that reconnects the mind, body, and soul to nature and normal common practical senses.

The moment health-seekers come into this new form of correct eating they are offered water from our water diversity stock; ozone water, distilled water, filtered water, living water, or herbal infused water. With a Nutritionally Diverse Meal, a Nutritionally Diverse shake is a great compliment. Different Kombuchas and cultured drinks are also important facets to this super diet concept.

Developing recipes with a larger range of ingredients is just as it seems, it is more complicated, but can provide more nutritional coverage and we believe it is the key to the next level of health and performance. I recommend having a few complicated fuller spectrum recipes for later in the day and then having the keep it simple combinations as I call them, or even one plant at a time during the workday, although this method requires sort of all-day snacking.  Smoothies and blended drinks are a great way to make the Nutritional Diversity concept more convenient.

Nutritional Diversity diet is developed across several permaculture farms and wildlife areas, and in a study and education center in Panama and one in Costa Rica also. The educational center is equipped with a cafe, and a few models for permaculture gardens that supply the bio-diverse foods to try their “garden to table.” This is the way food should come to the table, same-day harvest fresh, and five-minute harvest fresh is the best. Refrigeration does prolong freshness time.

The sustainable garden to table nutritionally-diverse cafe, and restaurants with the Ultimate Nutrition, will be the future choice of the future champions no doubt about it. If I had one here in the town that I write this from I would be there daily.

There are important social dynamics to alternative human optimization focused culture, for example, most regions in Asia revered symbols of balance and strong points of discipline that were roots of the cultures. Natural social growths that assist the cultivation of optimal men. The more ancient cultures of the Americas also featured many practices and representations in arts that fortified their prosperity and success. After a few failed attempts at setting up one of our own restaurants, we came up with a new program to help others do it and have a working model for the empowerment of the financially smaller individual.

The number one factor we have encountered to be wary of is selfish dishonest people that try to use others rather than uplift them in a never-ending cycle of communities working together, who seem to now, unfortunately, dominate the social-scape of our current time. I mention what I call ” the selfish disease” because it has been an incredible reoccurring factor in almost every one of our experiments. If we include anyone with this condition (92% of people have it), the endeavor will fail and we believe this experimental factor’s inclusion will cause any positive endeavor of this magnitude to fail.

Turns out nature itself has zero respect for the dishonest extremely selfish individual. Unfortunately in our experience once the disease has set in it is almost impossible to cure the subject of it.

The Next Big Entrepreneurship that both changes lives and helps nature.

There is a business model here for those who want to deliver the most awesome nutritional products – literally. Now that we know how to optimize human performance in every way, we almost have a responsibility to work in that interest. Never the less those who want to work in diverse nutrition, can start small and deliver the best nutritional products available, in a dream of an any-size business model.

Today I am going to bounce the idea off a successful friend to see if it finally clicks against what I perceive to be a very special and potential mind, that I met from another friend I consider to be of the same feather. In my life-long “keep the circle small” and “build organically” style, it’s also been important to really learn this over a decade with a diverse set of athletic and agricultural experiences. One of the best things today about the nutritionally diverse sciences is that it really has that real experience behind it now with scientific test results,

To continue that important note with an equally important thought; this year will be as each passing year of the last nine years in that we will venture deeper into the journey of nature taking it all in, getting to know better our species, and getting to know new species and specialties on the ride. The team today is very proud and happy to be living true lives, feeling real feelings, and having real experiences in a more natural way each passing year on this road. I don’t think anyone who is truly committed thus far has wanted to change their trajectory at all.

We have several drink recipes at the cafe with around 65 species, all organically grown and totally comprised of our own stuff here at our farm, grown by us. These drinks can be combined with meal options, to grant near 30 organic species to the consumer with outstanding taste! Should clients make a lunch and dinner plan with us, they can achieve the 60 species minimum Eisler described in his nutritionally diverse theory.

Delivery will be a big part of this and for farms like ours who produce we can deliver our species and produce in all stages of preparation and freshness. It has been a successful venture so far with a lot of real healing and helping people and a lot of loyal interest as well.

This is a great small business model being that as the food toxicity information in addition to the nutritional diversity information gets out there more and more, demand will grow for this kind of nutrition.

We call it presidential food, and we have recently acquired serious interest from the upper class in our areas of operation that seem hopeful.

Imagine if tomorrow an existing food outlet, such as the Subway food franchise started offering these alternatively grown non-toxic greens at even a small increase in price, the lines would be very long there. The grocery stores right now do not carry these things. They carry the centrally distributed food that is toxic.

From dreaming it to doing it..

ND Diet Developer, and the First Sustainable Nutritionally Diverse Cafe’s Resident Chef + The Leaves!

Eating at our nice little cafe/restaurant here in Panama folks can get to know what better nutrition is from the front lines. The cutting edge of nutrition can be in a business you start just like ours here. New Nutritional Diversifists, chef’s and developers of the diet can link with us now on Facebook and Instagram as well as right here in our own forum – that one is for serious interests that we can talk to a bit and feel out. We are building quite the team behind all of this and if interest exists that would like working on this more please advise how.

The Menu development realizes the availability of different like tasting things so the menu can stay tasting good while swapping ingredients.

Burritos are a good way to hold a bunch of stuff together without trying to have so much in the recipe that sticks it together such as with the veggie burger or multiple meat burger.

Stir Fry is the next obvious one that, could be easy to build on. Sheppard’s pies, vegetable lasagna’s and the like, but the best for me is the fresh salad.

There is nothing to it but to do it.

We have red meats, fish, and chicken models ready to go just shy of a few puzzle pieces to the final picture yet.

The concept is a bit of an explanation and it will take some serious work to get a nice little operation going with a good diversity of home-grown goodness. It’s a challenge but this diet and all the learning and connecting with nature that is involved can really be a fun endeavor.

One good vegetarian menu item we had done previously that went over very well a 13 different leaf mix cooked in coconut oil. Stems and all perfectly done up and lined with a nutritionally diverse pesto comprised of other rare ingredients and slightly fermented for a zesty kick, rolled up in a yucca flour tortilla.

Another was our leaf lasagna.

Highly Diverse salads are a great way to refuel the day.

When red meats are raised correctly the nutritional potential is immense. A nutritionally diverse diet works wonders on animals too. Goat meat is famous for its punch and this is largely because of the way goat farmers use goats to eat everything – which they do! Modern cuisine still though does not capitalize on the complete resource of the animal and a nutritionally diverse restaurant could offer a more complete “nose to tail” type of preparations for a more complete feast. There is almost a crippling of our system when only a fraction of their system is consumed. This type of thinking also motivates a more responsible and direct relationship to the life of the animal.

For a restaurant to invest in and manage its own farming operations could give it a great dynamic uniqueness that separates her from the competitors. For a restaurant to simply switch to a small farming ingredient(s) source(s) is a great way to close the loop for those food service businesses that are not ready to invest in their own agriculture.

As more of an agriculturist at the moment myself, what I have been doing is proposing the idea to existing restaurants of supplying the exotic species I grow, providing a few menu item recipe examples, and asking that they offer the plates or drinks at their location. A couple of menu items of exotic species from the new health culture is a good starting place for this concept to start working its way into societal availability. There also exists co-promotional opportunities and developmental possibilities in this type of arrangement, that be a great way for people who don’t have the investment capital or connections to start their own restaurant or cafe.

Join us on the all-natural human optimization mission to save the world!

Beyond Diverse Nutrition

More than beyond, it’s a deeper lesson in the nutritional study itself; that a certain level of knowledge and craftsmanship along with a certain level of character and honesty are required for both optimal biologic and metaphysic performance areas.

This morning, as I make some updates to this abstract information, on this new moon cycle of time, drinking an Artemis, Damiana, Mango, and pre-workout supplement drink, texting a few motivational messages to select friends in my area of Chiriqui for this couple of weeks here pushing my new Ju Jitsu coach of this region to hold class when he may not have today otherwise and proposing to what I can tell already is my new homie-in-arms to go see a Boxing coach in a nearby city who I like a lot; a very close friend of mine who I have trusted like I trust no other person at this time messaged me early about the three sisters Native American farming technique. I knew this was gonna be a good week the moment I woke up this morning!

No matter the girl who slept next to me, who is still sleep[ing now at 9a,m, no importance most of the culture here in Panama gets off to a late start, no-thing should ever be able to take away my strongest weapon in life – getting up very early and going to bed very early. My discipline, my daily push-ups, burpees, and workout routines are more so for my mind and spirit’s strength than any other part of me. There is no gym required for this, by the way, there is no excuse that works for this – there is only doing it.

Another friend who I have trusted as I trust no other at this point in time (timing is everything remember), did their job as a real friend and harped on me daily – “just get one thing done a day,” and “focus man, focus!”  He sees how the parental alienation voodoo confuses me every day, and how I can’t focus no matter how many herbs and super diet ingredients I may take per day, or how much I run or swim I still may not sleep well. So many of us now are bombarded by unnecessary and confusing dramas, voodoo attacks that may even use children and debt stresses, pandemic stresses, and are rendered less effective in life. I think that a realization of these things and how this nutritionally diverse diet concept can help them in addition to the importance of fitness should be two key importance to the diet concept’s culture and cultivation moving forward. These two concepts should be regarded as equally large as the agricultural requirements of permaculture or biodynamic agriculture as new food sources.

I am very thank full for all these valuable friendships for their guidance, positive influences, and for watching my back. This dynamic social function is also a key to prosperity and high quality of life in most social success studies. I am lucky to come from a town where my social stock (Albuquerque, New Mexico – in the 90’s, let’s not forget timing is everything) was very loving and protective of one another. In fact, one couldn’t peep a bad word about a guy at that time, in that town without risking their neck for it. We had a strong street code there and took a lot of pride in our differences from other cities in the U.S., we really had a higher bar of standards, and looking back after lots of diverse travel experience we were able to do a lot more with a lot less because of that.

These social dynamics are very important and many problems holding us back are related to our separation from nature and natural social interaction. After all this time our social culture lacks emphasis that should be present naturally such as the importance of humility and sobriety, dedication and discipline, or focus and honest social behavior to achieve success. Interestingly influence from media and social groups is highly negative and pointed to limit human performance.

It would be a missed opportunity if the whole new way of “eating food,” or “consuming nutrition” emerges among the strong, selected, organic righteous pioneers of truth and true health and performance without featuring dynamic cultural strong points.

So you see the Nutritionally Diverse Cafe & Restaurant is not just a small business, it is a free sustainable culture available to everyone that is separate from the modern toxic food supply.

This is a good step for now.

Eventually, we will grab ahold of Guerilla Permaculture and ideas such as; that we don’t even need to do a cafe or agriculture but simply to share the information for a justly karmic return, to fulfill our roles and true destinies in the eyes of nature.

To educate is to enable.

I met a guy at my hotel yesterday that I am pretty sure is going to be one of the ND team, of course, I could have never seen that in him, I could have never said anything to him, and right now I could choose not to have the faith that he will be.

I could be blind to the fact that my man, my killer amigo who is now shooting down for an epic meet-up with me now and I will be creating a masterpiece now years in the making – but the fact that I am not blind to that will only enhance and optimize that output.

Sincerely,

Your Friend

I have many names because I have many small jealous-minded stalkers and I try to keep my house clean no matter how many pathetic fleas try to dirty it up with their specs.

Remember there is physical size and growth, mental size and growth financial size and growth, and spiritual size and growth. Keep in mind what is most important to you.

Soon.

 Originally Published on Aug 13, 2018 at 01:01